Fiches Laboratoire

Molecular enzymology team :
- Methionine sulfoxide reductase (Msr) ;
- Disulfide oxidoreductase with folding thioredoxin/DsbE ;
- Aldehyde dehydrogenase with NAD(P) (ALDH) cofactor ;
- Sulfiredoxins (Srx).

RNA maturation team :
- The maturation of ribosomal RNA of S. cerevisiae yeast ;
- The formation of pseudouridine residue in S. cerevisiae ;
- Ribonucleoproteic particles of archaea guiding and catalysing the modification of ribosomal RNA ;
- The maturation of pre-messenger eukaryotic nuclear RNA ;
- The regulation of the splicing of the HIV-1 Virus ;
- The role of the SMN complex ;
- Computer research of genes coding small RNA in archaea ;
- Computer prediction of the 3D structure and the reactivity of RNA ;
- Structural and functional studies on the sur Xist RNA.

Determination of RNA sequences, including the identification of modified nucleotides.
Experimental determination of the 2D structure of RNA and their interaction site with proteins.
3D modelling of RNA and their reactivity by computer science approaches.
In vitro splicing techniques, application to the study of splicing regulations of the RNA of the HIV-1 virus.
Yeast genetics techniques (production of conditional mutants, double hybrid, triple hybrid, yeast cloning), applications to the study of RNA maturation mechanisms.
Enzymology techniques (stopped-flow, quenched-flow, fluorescence, characterization of the active site of enzymes by 2nd order and affinity markers).
Biophysical chemistry techniques: circular dichroism, microcalorimetry (ITC, DSC), fluorescence and fluorescence anisotropy.
Genetic engineering and proteic engineering (production and purification of recombinant proteins, site specific mutagenesis, remodelling).
Determination of 3D structures of proteins/enzymes or protein-enzyme/ligand, protein/protein/RNA complexes by XR and NMR.